Journal: iScience

Article Title: IPO5 contributes to cetuximab resistance in colorectal cancer by modulating Ras signaling

doi: 10.1016/j.isci.2026.117714

Figure Lengend Snippet:

Article Snippet: The primary antibodies used in this study were as follows: anti-IPO5 (1.0 μg/mL, Bioworld, China, #BS7341), anti-GAPDH (0.2 μg/mL, Proteintech, USA, #10494–1-AP), anti-AKT (1.0 μg/mL, Bioworld, China, #AP0489), anti- p -AKT (1.5 μg/mL, Bioworld, China, #BS4255), anti- p -EGFR (1.0 μg/mL, Bioworld, China, #BS6352), anti-active Caspase3 (1.5 μg/mL, Bioworld, China, #BS7004), anti-MEK1/2 (0.8 μg/mL, Affinity, USA, #AF6385), anti- p -MEK1/2 (1.0 μg/mL, Bioss, Beijing, #bsm-55741 R), anti-BCL-2 (0.5 μg/mL,Proteintech, USA, #12789-1-AP), anti-RASAL2 (1.2 μg/mL, Immunway, USA, #YN1852), anti- p -ERK (1.0 μg/mL, Immunway, USA, #YP0101), anti-ERK (0.8 μg/mL, Immunway, USA, #YT1625), anti-BAX (1.0 μg/mL, Immunway, USA, #YT0455), anti-EGFR (1.0 μg/mL, Abcam, USA, #ab52894), and anti-HRAS (0.5 μg/mL, Abcam, USA, #ab191595).

Techniques: Recombinant, Virus, Lysis, CCK-8 Assay, Protein Extraction, Activation Assay, Microarray, Gene Expression, shRNA, Sequencing, Control, Negative Control, Plasmid Preparation, Software

Journal: Cell Death Discovery

Article Title: p53 wild-type/low c-Myc-expressing ONS76 pediatric medulloblastoma cells become senescent after gamma-irradiation and can be eliminated by senolytic substances

doi: 10.1038/s41420-026-03404-4

Figure Lengend Snippet: ONS76 ( A ) and UW228-3 ( B ) cells were exposed to the indicated doses γIR and harvested at day 4, 5 and 6 after irradiation. Protein extracts were analyzed by western blotting for the expression of p53, p21, and Bcl-2, as well as actin as a housekeeper control. Please note that the ONS76 sample at day 5 after 9 Gy γIR was included on the UW228-3 blot ( B ) for a better comparison of protein expression levels between these two cell lines. This demonstrates the much lower p21 protein expression in irradiated p53-mutated UW228 cells when compared to irradiated p53 wild-type ONS76 cells. ( C–E ) ONS76 cells were transfected with ON-TARGET plus siRNA pools directed against p53 or p21, or with a nontargeting pool (siCon) as a control. 24 h after transfection, cells were divided equally and either left untreated (day 0) or were irradiated with 16 Gy γIR. At days 3 and 5 after irradiation, the cells were harvested and analyzed by western blotting ( C ) and flow cytometrical determination of Zombie-violet-( D ) and GLF16-positive cells ( E ). For ( D, E ), the mean % of positive cells ± S.D. of three independent experiments is shown. Individual data points are displayed in the bar charts by small filled circles. ** p < 0.01, *** p < 0.001. For ( A–C ), the western blots shown are representative of three to four independent experiments. Densitometric analyses of these blots can be found in Supplementary Fig. ( A–F ).

Article Snippet: From Santa Cruz (Heidelberg, Germany) was the mouse monoclonal Bcl-2 antibody (clone C-2; # sc-7382; AB_626736).

Techniques: Irradiation, Western Blot, Expressing, Control, Comparison, Transfection